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KMID : 0861020150300050045
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Korea Journal of Herbology
2015 Volume.30 No. 5 p.45 ~ p.49
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Effect of 'DaoCao' on the inflammatory cytokines in Human Jurkat cell and THP-1 cell
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Lee Young-Keun
Kim Cheong-Taek
Roh Seong-Soo
Choi Hak-Joo
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Abstract
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Objectives: The aim of this study is to investigate anti-inflammatory activity using various extracts of rice straw (DaoCao) extract (RS).
Methods : To investigate the anti-inflammatory effect of RS, we examined the effect of RS on cytokines production on THP-1 cell. Cells were cultured in incubator (37℃, CO2 5%, 0.5% FBS-RPMI, 1X106 cells/ml). One hour after, Dermatophagoides pteronissinus (Dp., 10 ug/ml) was treated into cell and at 6 hour after, each different concentrations(0.1, 1 and 10 ug/ml) of RS were treated. The cells were incubated for 16 hours and harvest the supernatant. The levels of IL-4, IL-5, IL-6, IL-8, MCP-1 and TNF-αwere determined using a commercially available ELISA kit.
Results: We investigated whether RS has the inhibition of inflammatory response in Jurkat cells and THP-1 cells. RS suppressed secretion of IL-4, IL-5, and TNF-α induced by house dust mites in Jurkat cells. It showed significant effects for all concentrations. RS suppressed the increased expression of IL-6, IL-8 and MCP-1 after treatment with mite in THP-1 cells. These results suggest that RS may be used as a valuable agent for treating allergic diseases such as atopy due to its anti-inflammatory property.
Conclusions: RS showed significant biological activities with anti-inflammatory in the human T cells. These results suggest that RS may be used as a valuable agent for treating allergic diseases such as atopy due to its anti-inflammatory property. In terms of Korean traditional medicine, we expect the results to contribute to building of EBM (Evidence-Based Medicine).
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KEYWORD
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Rise straw extract, DaoCao, anti-inflammatory, allergy, Dermatophagoides pteronissinus
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